Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Average cell number, doubling time and cell doubling number ±SD obtained at different time points (48, 72, 144, 216 h) from ADMSCs cultured on 2D plastic surface or 3D fibrin-based gel prepared from PPP (n = 5). Cells were seeded at a density of 6000 cells/cm 2 in 3.5 cm Petri dishes.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Cell Culture

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) obtained from cells maintained on plastic surface or inside a 3D matrix prepared from PPP, at different time points (48, 72, 144, 216 h) (n = 5). Statistically significant differences are indicated as follows: ** p ≤ 0.01, **** p ≤ 0.0001.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques:

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Morphology of ADMSCs grown inside a 3D matrix prepared from platelet-rich plasma (PRP) ( a1 , a2 , a3 ; 100×) or PPP ( b1 , b2 , b3 ; 100×), at different time points (0, 72, 144 h). The initial round-shaped morphology (a1, b1; time = 0 h), is changed in a spindle, fibroblast-like morphology at 72 and 144 h ( a2 , b2 , a3 , and b3 ). Cells gradually populate the whole 3D fibrin-derived scaffold, spreading over the entire thickness. No morphological differences are observed between cells maintained in PRP or platelet-poor plasma (PPP) derived matrix.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Clinical Proteomics, Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Average cell number, doubling time and cell doubling number obtained at different time points (72, 144 h) from ADMSCs cultured inside a 3D fibrin-based matrix prepared from PRP or PPP (n = 3).

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Cell Culture

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Box plot illustrating differences in average cell number ( a ), cell doubling time ( b ), and cell doubling number ( c ) of cells maintained inside a 3D matrix prepared from PRP or PPP, at different time points (72, 144 h) (n = 3). No statistically significant differences are observed between the two culture conditions.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques:

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Morphology of adipose tissue derived stromal vascular fraction (SVF) cells (100×), expanded on the plastic surface ( a ) or within a 3D matrix prepared from PPP. ( b ) The cells were expanded until they reached about 90% of confluence on plastic (average 192 h). The morphology resembles what observed for ADMSCs cultures in the two different environments.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: The average number of cells and cell number fold increase obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP. The cells were counted when plastic cultures reached about 90% of confluency (average 192 h) (n = 3).

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Graph bars representing the average number of cells obtained from adipose tissue derived SVF cells expanded on plastic surface or inside a 3D matrix prepared from PPP ( n = 3). Statistically significant differences are indicated as follows: ** p ≤ 0.01.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Derivative Assay

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: Evaluation of ADMSCs growth on plastic surface or inside the 3D fibrin-based matrix prepared from PPP, by MTT assay at 48 ( a ), 72 ( b ), and 96 ( c ) hours. The number of cells seeded was 5000/well (blue bars) or 10,000/well (yellow bars). The metabolic MTT assay confirmed the results observed by direct cell count. 3D fibrin-based matrix is a suitable environment to stimulate ADMSCs’ growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001.

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: MTT Assay, Cell Counting

Journal: Cells

Article Title: Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

doi: 10.3390/cells9122578

Figure Lengend Snippet: ( a ) Comparison between FBS and allogeneic canine serum as a medium supplement for ADMSCs culture, by MTT assay (5000 cells/well; 72 h). When seeded on plastic (blue bars), supplementation of medium with 10% allogeneic serum induced a statistically significant stimulus to cell growth with respect to FBS. When cells were cultured inside a 3D fibrin-based matrix prepared from PPP (yellow bars), allogeneic serum, and FBS supplementation, or no supplementation in the culture medium, did not induce different cell growth. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 (n = 3). ( b ) Autologous serum as a substitute for FBS in medium supplementation for ADMSCs growth (5000 cells/well; 72 h). When cells were cultivated on plastic surface (blue bars), 10% autologous serum medium supplementation was an effective substitute for 10% FBS supplementation. When cells were seeded inside a 3D fibrin-based matrix (yellow bars), no serum supplementation, FBS or autologous serum supplementation induced comparable results. Statistically significant differences are indicated as follows: **** p ≤ 0.0001 ( n = 3).

Article Snippet: PPP 3D matrix , 27.7 ± 6.5 , 27.9 ± 5.2.

Techniques: Comparison, MTT Assay, Cell Culture

Journal: Cells

Article Title: iPSCs as a Platform for Disease Modeling, Drug Screening, and Personalized Therapy in Muscular Dystrophies

doi: 10.3390/cells8010020

Figure Lengend Snippet: List of key studies using Induced pluripotent stem cells (iPSCs) for disease modeling in case of muscular dystrophies.

Article Snippet: DMD/LGMD BMD , iPSC-human , 3D matrix differentiation to observe myofibers formation. Triple lineage constructs created with 70% myogenic cells and 30% vascular , Development of 3D hydrogel platform for muscle stem cell and myofibers formation , 2018 , Maffioletti et al. [ ] .

Techniques: Retroviral, Transduction, In Vivo, Injection, Functional Assay, Expressing, Transplantation Assay, Inhibition, Transfection, Plasmid Preparation, Marker, Generated, Patch Clamp, Fluorescence, Construct, Cell Culture

Journal: Cells

Article Title: iPSCs as a Platform for Disease Modeling, Drug Screening, and Personalized Therapy in Muscular Dystrophies

doi: 10.3390/cells8010020

Figure Lengend Snippet: List of key studies using iPSCs for gene correction in case of muscular dystrophies.

Article Snippet: DMD/LGMD BMD , iPSC-human , 3D matrix differentiation to observe myofibers formation. Triple lineage constructs created with 70% myogenic cells and 30% vascular , Development of 3D hydrogel platform for muscle stem cell and myofibers formation , 2018 , Maffioletti et al. [ ] .

Techniques: HAC Assay, Sequencing, Transfection, Expressing, Functional Assay, CRISPR, Electroporation, Knock-In, Mutagenesis

Journal: Bioengineering

Article Title: Role of Collagen in Airway Mechanics

doi: 10.3390/bioengineering8010013

Figure Lengend Snippet: Airway disease stiffness alteration and associated collagen change.

Article Snippet: Aging lung , Increased collagen and decreased elastin production by fibroblasts , 3D matrix model and lung tissue , Increased pulmonary stiffness Decreased compliance , Collagen fibers have higher stiffness and lower deformation.

Techniques: Mutagenesis, Control, Expressing, Membrane, Generated, Tomography, Synthesized

Journal: Nature reviews. Cancer

Article Title: Cancer cell motility: lessons from migration in confined spaces

doi: 10.1038/nrc.2016.123

Figure Lengend Snippet: Summary of confined cell migration modes

Article Snippet: Lobopodial , Adhesions required , High RHOA levels , Linearly elastic 3D matrix , Nonpolarized cortactin, PIP 3 , RAC1 and CDC42 , Efficient migration following CDC42 or RAC1 knockdown , 64,80.

Techniques: Migration, Pore Size, Inhibition, Knockdown, Activation Assay